Main
Wiesław Abramowicz, M.S.
Laboratory Diagnostician, Oncological Computational Scientist (personalised diagnostic, nutri-, epi-, transcriptomics, epi-, genomics, proteomics, metabolomics)
Achivements: * Improving the functionality of the clinical laboratory IT system; * Cost effective sellecting personalized transcriptomics targets by microarray for on- and off-label treatment of tumors like in WINTHER clinical trial (NCT01856296); *The only person in Poland who performs the test properly on BD™ Bruker MALDI Biotyper;
Employment
Implementation Specialist
MedLAN
Białystok, PL
Currently - 05/2025
- IT technical support provided to contractors;
- implementation of IT applications;
- creating and testing database software for web applications;
- implementation of the needs of contractors from the area of pathology, laboratory clinical genetics in Poland;
- use for HL7, P1 protocols, medical laws, implementation of recommendations of scientific societies (e.g. PTPat, PTGC);
- support for implementation and development IT departments in the field of laboratory medicine.
Laboratory Diagnostician
Clinical genetic laboratory
Łódź, PL
12/2024 - 10/2024
- improving the functionality of the laboratory;
- quality control of each stage of laboratory work in accordance with ISO15189;
- EMQN;
- GLP knowledge;
- capacity calculation of flow cells; supervision of the execution of the library and patient sample preparation;
- operation of five devices for NIPT (liquid biopsy – cfDNA), WES, WGS (DNBSEQ-G400 Sequencer, DNBSEQ-T7 Sequencer; SBS – Salus Nimbo, Pro, EVO)[up to 1.8 Gbp, 5.8 Gbp and 3 Gbp];
- cooperation with Professors;
- superb contact with the vendors (Salus, MGI, Altium Group– service and genetic representative);
- assistance in solving clients needs.
Laboratory Diagnostician – volunteering
Białystok Oncology Center – Laboratory Diagnostics Facility
Białystok, PL
04/2024 - 11/2023
- InfoMedica;
- hematology (Sysmex XN-1000, XN-550, ACL350);
- biochemistry (Cobas 6000, e411, u411, u701, Integra 400 Plus, ABL90 flex);
- microbiology (Vitek 2 Compact, BD™ Bruker MALDI Biotyper - the only person in Poland who performs the test properly, according to the manufacturer’s service);
- blood components distribution;
- improving the functionality of the clinical laboratory IT system.
Owner, Co-founder & CEO
WARZEM Wood & Stone, Solid Wood Artistic Carpentry Workshop .
Dąbrowa Białostocka, PL
12/2022 - 4/2021
- fundraising;
- process optimization (Agile);
- management, accountancy;
- website design and build with HTML5, CSS, JS, YAML, Github, RStudio;
- Google Analytics;
- photography and video making;
- graphic design and creation (Inkscape – vector graphics);
- video design and creation (Blender);
- creating solid wood furnitures (using the golden ratio);
- carpentry tools design and create;
- using natural oils (no siccative, no PFAS) – toxicological issues;
- chopping down trees;
Pharmaceutical Assistant
Pharmacy “Lobelia” Barbara Winsko.
Białystok, PL
09/2009 - 09/2008
- advising for patients in OTC medication;
- issuing medicines prescribed by a physician;
- accepting and placing orders;
- delivering medicines to other points of sale.
Professional membership
The Right to Practice the Profession in Laboratory Diagnostic. No 13786
KIDL
Warsaw
Currently - 01/2014
Research Experience
Transcriptomic profiling of a human kidney cancer cell line, Caki-2, after treatment of Ixazomib (on-label), Ursolic acid and jointly using microarray technology. (pre-clinical)
N/A
Department of Pharmaceutical Biochemistry, Medical University of Białystok.
8/2017 - 10/2013
- Budget management (successful negotiations with vendors within budget);
- Design of Experiments (DoE);
- Project manager and executive (wet lab, in silico analyses);
- Performed wet laboratory experiments (Agilent’s gene expression and miRNA microarrays of indirect transcriptome form total RNA (mRNA, lncRNA, lincRNA, sno/snRNA, ncRNA, miscRNA, miRNA), quality controls (pos/neg/spike-in), MTT drug testing (proteasome inhibitor, terpen), total RNA/DNA isolation, purification, quality, quantity (Bioanalyzer, NanoDrop), gene validation (qPCR, primer design, analysis))
- and in silico analyses using open-source programming/biostatistical R/RStudio with packages from cran, bioconductor, github repos (pre-processing, numerical validation awarness, glm, Bayesian, multivariate, pathway enrichment analysis).
On time delivering wet lab experiments (I take care of the details and I am very precise, successful troubleshooting), performing and precaution of misleading computational analysis.
DNA sequencing of clear cell renal cell carcinoma and healthy kidney area. (clinical case)
“Genome-wide methods in cancer genetics”. BASTION.
Department of Pharmaceutical Biochemistry, Medical University of Białystok.
- project co-executive (wet lab, in silico analyses);
- DNA sequencing (1000 genes) of human cancer and normal kidney using KAPA library. [with co-operation of Prof. Rafał Płoski, MD, PhD (Medical University of Warsaw).
12/2015 - 10/2014
In Silico
Biostatistic experience
Interests: pre-processing (raw signal, transformations), manifolds/topology, glm, gee, mixed models
N/A
N/A
- exploratory and confirmatory analyses;
- GLMs & Bayesian, mixed (limma, mmrm, lme4);
- R, RStudio;
- R packages (GO.db, pathview, RSubread, Gviz, biomaRt, plotly, ggplot, D3.js, igvR, etc.);
- R programming (visualizations, office documentation, pdf, rtf);
- data mining, regular expression, rest api;
- pre-processing (log, lowess, loess, quantile) of transcriptomics data;
- Reproducible tools (git, github, gitlab) and reporting (rmarkdown, bookdown, etc.);
- Pathway Enrichment Analysis (GO, KEGG, Reactome, WikiPathways, HumanCyc, clusterProfiler, rSEA);
- Functional Annotation;
- GATK, STAR, DESeq2;
- Multivariate Analysis (PCA, tSNE, UMAP, SONG, NMF, Ricci flow);
- Comparative Analysis (Venn diagram);
- Isobolograms;
- use of data sets (ncbi, google patents, etc.);
- use of FDA resources (CDER, CDRH, NCTR – mitochondial toxicity, MAQC/SEQC);
- Gene Expression Omnibus;
- numerical validation awarness;
- Linux command line;
- basics of MySQL;
- basics of Python (PyScripter, PyMol, PyLasso, Autodock/Vina);
- basics of machine Learning (Random forest, CatBoost, LightGBM, XGBoost, H2O);
- basics of neural Networks (TensorFlow, Keras, etc.);
- Agilent’s GeneSpring GX;
- CLC Main Workbench;
- more information in each description of course;
HPC experience
High-Performance and Parallel Computing
N/A
N/A
- I built cluster of two personal computers with openMPI and Rmpi (an interface for R & RStudio) on Ubuntu (SSH connected).
Wet Lab
Laboratory Experience
Research interests: Oncology, Immunology, EpiOmics, Transcriptomic, Proteasomes, Nutritranscriptomics, Watson-Crick/Hoogsteen base paring.
Medical University of Białystok, PL
2017 - 2011
- BIO-RAD CFX Connect Real-Time PCR (qPCR);
- Agilent’s microarray (two-color gene expression, miRNA) + QC (positive, negative, spike-in controls);
- NanoDrop 2000 (spectrophotometry);
- MTT tests (using proteasome inhibitor {Ixazomib} – on-label FDA approved in multiple myeloma, terpen {Ursolic acid} – derivative, from e.g. apples, with anti-inflammation, anti-cancer properties);
- DNA, RNA extraction from cultured cells (Qiagen, Promega, A&A Biotechnology); RNase, DNase free environment;
- Agilent 2100 Bioanalyzer (DNA/RNA microcapillary electrophoresis, flow cytometry) – lab-on-chip;
- DNA, RNA gel electrophoresis;
- Western blot with SNAP i.d.;
- PMN, PBMC cells isolation, neutrophils isolation with anti-CD16 mAb MicroBeads and magnetic separator Midi MACS;
- cells counting chambers;
- setting up cell cultures;
- optical microscope;
- flow cytometry.
Education
Data Science Specialization
coursera.org – online
Johns Hopkins University, USA
Currently - 01/2016
“Exploratory Data Analysis” (11/2018)
- lattice.
- ggplot2.
- Clustering.
- Dimension Reduction.
“Getting and Cleaning Data” (02/2016)
- dplyr, httr, rhdf5, xml, RMySQL, foreign, rmongodb, RMongo, RPostresSQL;
- Regular Expressions (REGEX), R programming Data Cleaning, Data Manipulation.
“R programming” (01/2016)
- Data Analysis, R programming, Debugging, RStudio.
“The Data Scientist’s Toolbox” (01/2016)
- Version Control (Git, GitHub).
- R Markdown.
Ph.D. Studies in Pharmaceutical Sciences
The Center for Innovative Research 2012-2017, Faculty of Medicine with the Division of Dentistry and Division of Medical Education in English, Faculty of Pharmacy and Division of Laboratory Medicine, Medical University of Białystok
PL
08/2017 - 10/2013
Research area: Transcriptomic profiling of cancer cell line after drugs treatment using Agilent microarrays and biostatistic open-source tools.
Advisor: Prof. dr hab. Marzanna Cechowska-Pasko, Ph.D.
All subjects passed (GPA 3,85):
- Principles of genetic analysis (6h);
- Learning techniques of molecular biology (10h);
- Introduction to genomics (6h);
- Epigenomics and transcriptomics (50h);
- Learning methods in structural and functional genomics (10h);
- Basic Statistical refreshment (30h; prof. Tomasz Burzykowski, UHasselt);
- Statistical modeling (30h; prof. Tomasz Burzykowski, UHasselt);
- Design of experiments (18h; prof. Tomasz Burzykowski, UHasselt);
- Statistics for omics (36h; prof. Ziv Shkedy, UHasselt);
- Metabolomics (16h);
- Facultative advanced courses in protein analysis/ proteomics/ metabolomics (50h);
- Protein analysis and proteomics (32h);
- Immunology (16h);
- Facultative advanced courses in immunology (15h);
- Teaching the presentation and evaluation of research at the academic level (30h);
- Professional practice (30h);
- OHS (4h).
M.Sc. in Medical Analitics, PQF 7
Faculty of Pharmacy and Division of Laboratory Medicine, Medical University of Białystok
PL
09/2013 - 10/2007
Master’s thesis: Expression of the APRIL particle in neutrophils of patients with potentially malignant lesions of the oral mucosa.
- GPA: 4,30 (transcript of master degree);
- isolation and work with human blood cells;
- Advisor: Prof. dr hab. Ewa Jabłońska, Ph.D.
- Co-Advisor: dr Kamil Grubczak, Ph.D.
- experience in biochemistry and immunology research (see Wet lab, Student membership & Publications sections in this resume);
- Dean’s leave (10/2008 - 09/2009)
Pharmaceutical Assistant, PQF 4
Vocational School No. 1 of Health Care
Białystok, PL
08/2007 - 09/2005
Data Science, Laboratory Courses
Duke Industry Statistics Symposium 2023
“Empower Clinical Development by Harnessing Data from Diverse Sources”
Duke University, USA
29/ - 31/03/2023
“Good Things Come in Small Packages: Purifying miRNA from Plasma, Serum and Exosomes”
Michelle Mandrekar – Promega
Online, PL
25/01/2023
- Maxwell RSC miRNA
Courses:
A&A Biotechnology
Gdańsk, PL
22/ - 25/09/2014
- “Real-Time PCR”
- “Real-Time PCR - quantitative markings”
Prof. Tomasz Motyl Laboratory Experience:
Department of Physiological Sciences, WULS (SGGW)
Warsaw, PL
10/2014
- Agilent’s expression microarray experiments (wet lab only).
“NGS in Regulatory Gene Research”
ideas4biology
Poznań, PL
06/2016
Coordinator: dr Michał Szcześniak, Ph.D.
Techniques: ChIP-chip, ChIP-Seq, ChIP-exo, CLIP-Seq, DNase-Seq, FAIRE-Seq, GRO-Seq, HiC, MeDIP-Seq, MBD-Seq, MNase-Seq, oxBS-Seq, RIP-Seq, RRBS-Seq, TAB-Seq, TSS-Seq, WGBS-Seq.
Linux. WinSCP, PuTTY.
Softwares: POLYPHEMUS, ChromHMM, Segway, MACE, DANPOS2, MeDUSA, diffReps, BSMAP, methylKit, Trimmomatic, Subread, BamTools.
Databases: OMICtools, AllSeq, ChIPBase, hmChIP, mirPath, miRTarBase.
“Using next-generation sequencing to analyse human transcriptome”
Richard Dixon, Ph.D. – QIAGEN
Medical University of Bialystok, PL
16/ - 18/06/2014
“Using next generation sequencing to analyse human genome”
Richard Dixon, Ph.D. – QIAGEN
Medical University of Bialystok, PL
08/ - 11/04/2014
“Early diagnosis and treatment of neurodegenerative diseases”
Department of Biochemical Diagnostics, Department of Neurology, Department od Neurodegeneration Diagnostics, Department of Pediatrics, Gastroenterology and Pediatric Allergology of Medical University of Bialystok
Bialystok, PL
07/04/2014
Student membership
Member of the board of the Students’ Scientific Society
Co-organizer of the 8th & 7th Białystok International Medical Congress for Young Scientists. Coordinator of Belarusian participants.
Medical University of Białystok, PL
2013 - 2012
Department of Immunology
Active participant of Student’s Scientific Society.
Medical University of Białystok, PL
06/2013 - 10/2009
Advisor: Prof. Ewa Jabłońska, Ph.D.
Department of Pharmaceutical Biochemistry
Active participant of Student’s Scientific Society.
Medical University of Białystok, PL
2011 - 2010
Advisor: dr hab. Małgorzata Borzym-Kluczyk, Ph.D.
Publications and Posters
Tylirozyd, a flavonoid with cytotoxic properties. Poster presentation.
Nationwide Scientific Symposium “Supplements a deity under the microscope of a pharmacist”.
Medical University of Bialystok, PL
2013
Abramowicz W.
APRIL molecule in patients with potentially malignant lesions of the oral mucosa (lichen planus).
7th International Medical Congress for Young Scientists, Białystok, p.185
N/A
2012
Abramowicz W., Wawrusiewicz-Kurylonek N., Garley M.
Alpha-mannosidase and beta-galactosidase activity in the serum of patients with colorectal adenocarcinoma.
5th International Scientific Conference of Medical Students and Young Doctors, Białystok, p.132-133
N/A
2010
Luto M., Choromańska B., Chwiła A., Wasiluk A., Abramowicz W.
Assessment of the activity of N-acetyl-beta-D-hexosaminidase, it’s B enzyme and betaglucuronidase in the urine of patients with Lyme disease.
5th International Scientific Conference of Medical Students and Young Doctors, Białystok, p.127
N/A
2010
Choromańska B., Luto M., Abramowicz W., Chwiła A., Wasiluk A.
The activity of N-acetyl-beta-hexosaminidase, it’s N-isoenzyme and beta-glucuronidase in the blood serum of patients suffering from Lyme disease.
5th International Scientific Conference of Medical Students and Young Doctors, Białystok, p.128
N/A
2010
Chwiła A., Luto M., Choromańska B., Wasiluk A., Abramowicz W.
Alanine and aspartic aminotransferase activity in blood serum of men after acute and chronic ethyl alcohol poisoning.
5th International Scientific Conference of Medical Students and Young Doctors, Białystok, p.137-138
N/A
2010
Wasiluk A., Abramowicz W., Luto M., Choromańska B., Chwiła A.
Driving licence
AM/B1/B
Smooth driving, dynamic driving, no collisions.
N/A
Currently - 30/08/2006
Disclaimer
Last updated on 2025-08-04.